Human TLR7 Stable Cell Line

Name	Human TLR7 Stable Cell Line
Supplier	Novus Biologicals
Catalog	NBP2-26270
Category	Cell Line
Prices	$849.00
Sizes	1 vial
Gene	TLR7
Species	Human
Growth Mode	Adherent Morphology: Epithelial
Description	The TLR7 stable cell line can be used for TLR7 flow cytometric calibration and detection control as well as TLR7-dependent functional assays. TLR7 expression in this stable cell line has been validated by Western blotting (fig. 1) and flow cytometry (fig. 2). Functional activity of this stable cell line has been validated using the NF-kB/SEAPorter™ Assay Kit (NBP2-25286, fig. 3). Contents: 3~4 x 10^6 cells Biosafety Level: 2
Supplier Page	Shop

Product images

Western Blot: Human TLR7 Stable Cell Line [NBP2-26270] - analysis of TLR7 expression in the TLR7 stable cell line using an HA antibody (20 ug total protein/lane). Legend. Vect: Vector control stable cell line; TLR7: TLR7 stable cell line.

Flow Cytometry: Human TLR7 Stable Cell Line [NBP2-26270] - analysis of TLR7 expression in the TLR7 stable cell line. Intracellular expression of TLR7 in the TLR7 stable cell line was analyzed by flow cytometry using a FITC-conjugated TLR7 antibody and compared with the Vector control stable cell line. Intracellular TLR Staining Flow Kit was used for this test.

Functional: Human TLR7 Stable Cell Line [NBP2-26270] - Functional analysis of the TLR7 stable cell line. The assay was performed using the NF-kB SEAPorter™ Assay Kit (NBP2-25286). The Vector control stable cell line (NBP2-26259) and TLR7 stable cell line were transfected with NF-kB/SEAP reporter plasmid for 16 h. Cells were stimulated with Imidazoquinoline resiquimod R-848 for 24 h followed by SEAP assay.